Fine Needle Aspiration Biopsy Cytopathology of the Breast Utilizing Liquid-Based Preparations

Abstract

The liquid-based cytology (LBC) technique is an automated method for preparing thin-layer cytological samples from cell suspensions collected in alcohol-based preservative. LBC preparations of breast fine needle aspiration biopsies (FNAB) demonstrate better cellular preservation, less cell overlapping, and elimination of excessive blood and inflammation compared with conventional smears (CS). Conversely, alterations in architecture and cell morphology as well as loss of myoepithelial cells and stromal elements have been described in LBC preparations. Similar accuracy for LBC and CS has been demonstrated in different studies. However, LBC requires specific training before applying this technique. LBC is suitable for ancillary testing. This chapter presents the general cytomorphological features of breast FNAB in LBC and then the findings in specific lesions.