Zeta potential of human X‐ and Y‐bearing sperm

Abstract

The zeta potential of human X‐ and Y‐bearing sperm was measured by two different methods: (i) using an electrophoretic light scattering spectrophotometer, and (ii) using a laser‐rotating prism. The X‐ and Y‐bearing sperm were separated by free‐flow electrophoresis, and their purities were determined by staining for the F‐body using quinacrine mustard. The zeta potential of the sperm in the fraction containing more than 80% Y‐bearing sperm was approximately ‐16 mV, whereas that of sperm in the fraction containing more than 95% X‐bearing sperm was approximately — 20 mV. In other words, the net negative‐charge on the cell surface of human X‐bearing sperm is higher than that of Y‐bearing sperm. Copyright © 1991, Wiley Blackwell. All rights reserved