In vivo imaging of hepatic neutrophil migration in severe alcoholic hepatitis with 111 In-radiolabelled leucocytes

7Citations
Citations of this article
13Readers
Mendeley users who have this article in their library.

Abstract

The study’s aim was to image severe alcoholic hepatitis (SAH) using 111 In-labelled leucocytes with two objectives in mind: firstly for non-invasive diagnosis and secondly to provide a platform for experimental therapies aiming to inhibit intrahepatic neutrophil migration. 111 In-leucocyte scintigraphy was performed 30 min and 24 h post-injection in 19 patients with SAH, 14 abstinent patients with alcohol-related cirrhosis and 11 normal controls. Eleven with SAH and seven with cirrhosis also had 99m Tc-nanocolloid scintigraphy. Change in hepatic 111 In radioactivity was expressed as decay-corrected 24 h:30 min count ratio and, in SAH, compared with histological grading of steatohepatitis and expression of granulocyte marker, CD15. Hepatic microautoradiography on biopsy specimens obtained 24 h post-injection of 111 In-leucocytes was performed in one patient. Median 24 h:30 min hepatic 111 In activity ratio was higher in SAH (2.5 (interquartile range (IQR): 1.7–4.0) compared with cirrhotics and normal controls (1.0 (0.8–1.1) and 0.8 (0.7–0.9) respectively, P<0.0001). In SAH, it correlated with CD15 expression (r = 0.62, P=0.023) and was higher in marked compared with mild/moderate steatohepatitis (4.0 (3.0–4.6) compared with 1.8 (1.5–2.6), P=0.006). Hepatic-to-splenic 99m Tc count rate ratio was reduced in SAH (0.5 (0.4–1.4)) compared with cirrhotics (2.3(0.6–3.0)) and three historic normal controls (4.2 (3.8–5.0); P=0.003), consistent with impaired hepatic reticuloendothelial function. Scintigraphic findings in SAH included prominent lung radioactivity at 30 min, likely the result of neutrophil primimg. Microautoradiography demonstrated cell-associated 111 In in areas of parenchymal neutrophil infiltration. In conclusion, 111 In-leucocyte scintigraphy can non-invasively diagnose SAH and could provide a platform for evaluation of novel treatments aiming to inhibit intrahepatic neutrophil migration.

Cite

CITATION STYLE

APA

Potts, J. R., Farahi, N., Howard, M. R., Taylor, M. R., Heard, S., Shankar, A. N., … Peters, A. M. (2018). In vivo imaging of hepatic neutrophil migration in severe alcoholic hepatitis with 111 In-radiolabelled leucocytes. Bioscience Reports, 38(4). https://doi.org/10.1042/BSR20180466

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free