SOS mutagenesis in Escherichia coli requires the functions of the umuD, C genes, or their functional analogues mucA, B derived from a plasmid pKM101, and the recA gene. However, mere derepression of these SOS genes does not increase the ability of the cell to perform mutagenesis. Activation of RecA protein to a form (RecA*) that mediates cleavage of the LexA repressor is required for mutagenesis. We present evidence that UmuD and MucA are proteolytically processed by RecA* and that the processed products are the active forms involved in mutagenesis.
CITATION STYLE
Shiba, T., Iwasaki, H., Nakata, A., & Shinagawa, H. (1990). Proteolytic activation of UmuD and MucA proteins for SOS mutagenesis. Basic Life Sciences, 52, 351–354. https://doi.org/10.1007/978-1-4615-9561-8_31
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