Background: Gene dosage change is a mild perturbation that is a valuable tool for pathway reconstruction in Drosophila. While it is often assumed that reducing gene dose by half leads to two-fold less expression, there is partial autosomal dosage compensation in Drosophila, which may be mediated by feedback or buffering in expression networks.Results: We profiled expression in engineered flies where gene dose was reduced from two to one. While expression of most one-dose genes was reduced, the gene-specific dose responses were heterogeneous. Expression of two-dose genes that are first-degree neighbors of one-dose genes in novel network models also changed expression, and the directionality of change depended on the response of one-dose genes.Conclusions: Our data indicate that expression perturbation propagates in network space. Autosomal compensation, or the lack thereof, is a gene-specific response, largely mediated by interactions with the rest of the transcriptome. © 2012 Malone et al.; licensee BioMed Central Ltd.
CITATION STYLE
Malone, J. H., Cho, D. Y., Mattiuzzo, N. R., Artieri, C. G., Jiang, L., Dale, R. K., … Oliver, B. (2012). Mediation of Drosophila autosomal dosage effects and compensation by network interactions. Genome Biology, 13(4). https://doi.org/10.1186/gb-2012-13-4-r28
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