In this chapter, we describe the minigenome system for Ebola virus (EBOV), which reconstitutes EBOV polymerase activity in cells and can be used to model viral genome replication and transcription. This protocol comprises all steps including cell culture, plasmid preparation, transfection, and luciferase reporter assay readout.
CITATION STYLE
Cressey, T., Brauburger, K., & Mühlberger, E. (2017). Modeling ebola virus genome replication and transcription with minigenome systems. In Methods in Molecular Biology (Vol. 1628, pp. 79–92). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7116-9_6
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