The determination of kinetic information and appropriate binding pairs is fundamental to the proper optimization and selection of ligands used in immunoassays, diagnostics, and therapeutics. However, the ability to estimate such parameters in a multiplexed and inexpensive format remains difficult and modification of the ligand is often necessary. Here, we detail the methods and materials necessary to evaluate hundreds of unlabeled ligands simultaneously using the interferometric reflectance imaging sensor (IRIS). The incorporation of a low-cost fluidic cartridge that integrates on the top of the sensor simplifies reagent handling considerably.
CITATION STYLE
Needham, J. W., Ünlü, N. L., Yurdakul, C., & Ünlü, M. S. (2019). Interferometric Reflectance Imaging Sensor (IRIS) for Molecular Kinetics with a Low-Cost, Disposable Fluidic Cartridge. In Methods in Molecular Biology (Vol. 2027, pp. 15–28). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9616-2_2
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