Bioluminescent imaging and tracking of bacterial transport in soils

Abstract

Bioimaging instrumentation can be used to observe environmental phenomena such as the transport, retention, and distribution of bacteria in soils in situ in a real-time, nondestructive manner. Bacteria designed to emit bioluminescence light signals are injected into a transparent column packed with soils, and then the column is placed into a bioimaging instrument, such as a PerkinElmer IVIS Spectrum, while it is connected through thin teflon tubes to other parts of the column system located outside of the imaging chamber, including a fraction collector for collecting effluent solution and a pump for introducing bacterial suspension or experimental solution. After self-correction of soil autofluorescence and bioluminescence and setup of required imaging parameters, the transport experiment is initiated by introducing the bacterial suspension to the soil column while the spatiotemporal distribution of bioluminescent bacteria in the entire soil column is imaged. Finally, the images are processed to analyze bacterial migration in the soil under various environmental conditions in comparison with the breakthrough and elution curves of the bacteria obtained by analyzing the effluent samples.