Diferentes suplementos no cultivo in vitro de embriões de pinhão-manso

Abstract

The objective of this work was to evaluate the influence of maturation stages of physic nut fruits on the development of their embryos cultivated in MS medium supplemented with sucrose, coconut water and activated carbon. Fruits were collected, and their embryos were extracted aseptically. They were submitted to two experimental conditions: embryos from fruits in three development stages (immature, mature and dry) were put into MS medium, supplemented with sucrose (0, 15, 30 and 60 g L-1); embryos derived from dry fruits were put into MS medium supplemented with sucrose 30 g L -1, activated carbon (0, 1, 2 and 3 g L-1), and coconut water (0, 50, 100, 150, 200 e 250 mL L-1). Explants were kept in a growth chamber under controlled conditions during 30 days. Only the zygotic embryos from immature fruits needed sucrose supplementation in the culture medium to sustain germination. Better embryos development occurs in MS medium supplemented with 60 g L-1 of sucrose. The association of activated carbon and coconut water provides better growth of seedlings derived from embryos extracted from seeds of dry fruits.