Combined immunocytochemistry and enzyme cytochemistry on ultra-thin cryosections: A new method

Abstract

We combined immunocytochemistry and enzyme cytochemistry to localize two different proteins on the same ultra-thin cryosection. In this method the immunocytochemical localization is visualized with colloidal gold probes and the enzyme cytochemical detection is achieved with cerium as the capture agent. The immunocytochemistry is conducted first so that any potential adverse effects of the enzyme cytochemical procedure will not alter the antibody binding properties of the cryosections.