Single-molecule atomic force microscopy (AFM) allows capturing the conformational dynamics of an individual molecule under force. In this chapter, we describe a protocol for conducting a protein nanomechanical experiment using AFM, covering both the force-extension and force-clamp modes. Combined, these experiments provide an integrated vista of the molecular mechanisms—and their associated kinetics—underpinning the mechanical unfolding and refolding of individual proteins when exposed to mechanical load.
CITATION STYLE
Mora, M., Tapia-Rojo, R., & Garcia-Manyes, S. (2024). Unfolding and Refolding Proteins Using Single-Molecule AFM. In Methods in Molecular Biology (Vol. 2694, pp. 339–354). Humana Press Inc. https://doi.org/10.1007/978-1-0716-3377-9_16
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