Comparisons of Pasteurella multocida lipopolysaccharides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine relationship between group B and E hemorrhagic septicemia strains and serologically related group A strains

Abstract

Lipopolysaccharides (LPSs) purified from 16 reference somatic serotypes of Pasteurella multocida were examined and compared by discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Resolution of LPS patterns in a gel was optimum when sample wells were cast separately from the stacking gel and the running gel consisted of 15% T (total monomer) polyacrylamide and 4 M deionized urea. Band patterns of P. multocida LPSs in a gel differed from control Salmonella minnesota wild-type and core mutant LPSs. Although the band patterns and mobilities of LPSs from some P. multocida reference serotypes were similar, none were identical. Evidence for O antigens similar to those produced by enterobacteria was not observed. Proteinase K digestion of whole P. multocida cells resulted in LPS band patterns similar to those of purified LPS. The presence or absence of a capsule on a strain had no major influence on band patterns in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Comparisons of LPS patterns of group B and E hemorrhagic septicemia strains with those of serologically related group A strains of P. multocida indicated that they were similar. Typing antisera made with purified serotype 2 or 5 LPS reacted with electroblots of all these strains. However, the reactions did not distinguish strains as being serotype 2 or 5.