A new pathway for poly(3-hydroxybutyrate) production in escherichia coli and corynebacterium glutamicum by functional expression of a new acetoacetyl-coenzyme A synthase

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Abstract

A biosynthetic pathway for poly(3-hydroxybutyrate) [P(3HB)] was developed in Escherichia coli and Corynebacterium glutamicum by an acetoacetyl-coenzyme A (CoA) synthase (AACS) recently isolated from terpe-noid-producing Streptomyces sp. strain CL190. Expression of AACS led to significant productions of P(3HB) in E. coli (10.5 wt %) and C. glutamicum (19.7 wt %).

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Matsumoto, K., Yamada, M., Leong, C. R., Jo, S. J., Kuzuyama, T., & Taguchi, S. (2011). A new pathway for poly(3-hydroxybutyrate) production in escherichia coli and corynebacterium glutamicum by functional expression of a new acetoacetyl-coenzyme A synthase. Bioscience, Biotechnology and Biochemistry, 75(2), 364–366. https://doi.org/10.1271/bbb.100682

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