Microarray-based immunoassay for parallel quantification of multiple mycotoxins in oat

Abstract

Because multianalyte methods are highly desirable in order to keep analysis time and costs low, the biosensor development increasingly focuses on parallel analysis of several mycotoxins. Here, we describe an indirect competitive immunoassay on regenerable, reusable glass microchips for the parallel determination of aflatoxins, ochratoxin A, deoxynivalenol, and fumonisin B1 in oat extracts, using a fully automated flow-­ through device with chemiluminescence readout.