Lack of mitochondrial and nuclear-encoded subunits of complex I and alteration of the respiratory chain in Nicotiana sylvestris mitochondrial deletion mutants

176Citations
Citations of this article
56Readers
Mendeley users who have this article in their library.

Abstract

We previously have shown that Nicotiana sylvestris cytoplasmic male sterile (CMS) mutants I and II present large mtDNA deletions and that the NAD7 subunit of complex I (the main dehydrogenase of the mitochondrial respiratory chain) is absent in CMS I. Here, we show that, despite a large difference in size in the mtDNA deletion, CMS I and II display similar alterations. Both have an impaired development from germination to flowering, with partial male sterility that becomes complete under low light. Besides NAD7, two other complex I subunits are missing (NAD9 and the nucleus-encoded, 38-kDa subunit), identified on two-dimensional patterns of mitochondrial proteins. Mitochondria isolated from CMS leaves showed altered respiration. Although their succinate oxidation through complex II was close to that of the wild type, oxidation of glycine, a priority substrate of plant mitochondria, was significantly reduced. The remaining activity was much less sensitive to rotenone, indicating the breakdown of Complex I activity. Oxidation of exogenous NADH (coupled to proton gradient generation and partly sensitive to rotenone) was strongly increased. These results suggest respiratory compensation mechanisms involving additional NADH dehydrogenases to complex I. Finally, the capacity of the cyanide-resistant alternative oxidase pathway was enhanced in CMS, and higher amounts of enzyme were evidenced by immunodetection.

References Powered by Scopus

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

232748Citations
N/AReaders
Get full text

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications

47832Citations
N/AReaders
Get full text

Differential staining of aborted and nonaborted pollen

1333Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Interactions of mitochondrial and nuclear genes that affect male gametophyte development

679Citations
N/AReaders
Get full text

Leaf mitochondria modulate whole cell redox homeostasis, set antioxidant capacity, and determine stress resistance through altered signaling and diurnal regulation

442Citations
N/AReaders
Get full text

Mitochondrial reactive oxygen species. Contribution to oxidative stress and interorganellar signaling

439Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Gutierres, S., Sabar, M., Lelandais, C., Chetrit, P., Diolez, P., Degand, H., … De Paepe, R. (1997). Lack of mitochondrial and nuclear-encoded subunits of complex I and alteration of the respiratory chain in Nicotiana sylvestris mitochondrial deletion mutants. Proceedings of the National Academy of Sciences of the United States of America, 94(7), 3436–3441. https://doi.org/10.1073/pnas.94.7.3436

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 18

41%

Researcher 15

34%

Professor / Associate Prof. 10

23%

Lecturer / Post doc 1

2%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 31

72%

Biochemistry, Genetics and Molecular Bi... 10

23%

Pharmacology, Toxicology and Pharmaceut... 1

2%

Computer Science 1

2%

Save time finding and organizing research with Mendeley

Sign up for free