γ-Aminobutyric Acid Type A Receptor Subunit α-6 (GABRA6) Gene Polymorphism and Anxiety Disorder

Abstract

Anxiety disorder caused by environmental factor and individual genetic variations. Gamma-aminobutyric acid type A receptors Subunit α-6 (GABRA6) is γ-aminobutyric acid type A (GABA) receptor. Single Nucleotide Polymorphism (SNP) of GABRA6 gene at rs3219151 (T1521C) affected individual response of stress. The aim of present study was to identify GABRA6 genotype variations in Bandung city population and its correlation with stress condition. Samples were collected from 112 respondents who filled The Kessler Psychological Distress Scale (K10) questionnaire for stress condition. Blood samples were collected and identification of GABRA6 gene was analyzed using Polymerase Chain Reaction‑Refractory Fragment Length Polymorphism (PCR-RFLP) by AlwN1 restriction enzyme digestion. The result of present study showed that 84 respondents (75%) have CC genotype, 14 respondents (12.5%) have CT genotype, and other 14 respondents (12.5%) have TT genotype. Most of respondents have CC genotype but the data did not meet the Hardy-Weinberg equilibrium and showed no correlation between GABRA6 gene variations and stress condition using bivariate analysis (Chi-Square).