Quantitative real-time PCR (qRT-PCR) is a common technique for mRNA quantification. Several methods have been developed in the past few years in order to adapt qRT-PCR also for small non-coding RNAs (sRNA). We here provide a simple and sensitive protocol that allows quantification of mRNAs, selected sRNAs, and long non-coding RNAs (lncRNA) in one cDNA sample by qRT-PCR.
CITATION STYLE
Speth, C., & Laubinger, S. (2014). Rapid and parallel quantification of small and large RNA species. Methods in Molecular Biology, 1158, 93–106. https://doi.org/10.1007/978-1-4939-0700-7_6
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