Cationic PLGA/eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint

24Citations
Citations of this article
55Readers
Mendeley users who have this article in their library.

Abstract

Positively surface-charged poly(lactide-co-glycolide) (PLGA)/Eudragit RL nanoparticles (NPs) were designed to increase retention time and sustain release profile in joints after intra-articular injection, by forming micrometer-sized electrostatic aggregates with hyaluronic acid, an endogenous anionic polysaccharide found in high amounts in synovial fluid. The cationic NPs consisting of PLGA, Eudragit RL, and polyvinyl alcohol were fabricated by solvent evaporation technique. The NPs were 170.1 nm in size, with a zeta potential of 21.3 mV in phosphate-buffered saline. Hyperspectral imaging (CytoViva®) revealed the formation of the micrometer-sized filamentous aggregates upon admixing, due to electrostatic interaction between NPs and the polysaccharides. NPs loaded with a fluorescent probe (1,1′-dioctadecyl-3,3,3′,3′ tetramethylindotricarbocyanine iodide, DiR) displayed a significantly improved retention time in the knee joint, with over 50% preservation of the fluorescent signal 28 days after injection. When DiR solution was injected intra-articularly, the fluorescence levels rapidly decreased to 30% of the initial concentration within 3 days in mice. From these findings, we suggest that PLGA-based cationic NPs could be a promising tool for prolonged delivery of therapeutic agents in joints selectively.

Cite

CITATION STYLE

APA

Kim, S. R., Ho, M. J., Lee, E., Lee, J. W., Choi, Y. W., & Kang, M. J. (2015). Cationic PLGA/eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint. International Journal of Nanomedicine, 10, 5263–5271. https://doi.org/10.2147/IJN.S88363

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free