Sensitive detection of multiple fluoresence probes based on surface-enhanced raman scattering (sers) for mers-cov

Abstract

Up to now, Middle East respiratory syndrome (MERS) is solely confirmed with polymerase chain reactions (PCR) that is limited to detect a single biomarker on a trial and take long resulting time. Meanwhile, plasmonic paper substrates are eligible as multiplex Surface enhanced Raman scattering (SERS) platform for sensitive detection of multiple dual-labeled PCR probes on single analysis. The plasmonic paper, which contains silver nanodot deposited on 3D cellulose fiber, allows for simultaneous identification and the scalable quantification of fluorescence dyes over broad concentration ranges. Besides, developed biotin-modified probes enable to precisely separate the multiple fluorophores from PCR end-product. The platform will reduce both the number of PCR cycle and analysis time, and be applicable for emergency point-of-care (POC) tests of infectious diseases. It could help to block spread of epidemic disease in a country or world. Furthermore, the rapid diagnostic method could make patients earlier recover from acute severe diseases.