Aestuariibacter aggregatus sp. nov., a moderately halophilic bacterium isolated from seawater of the Yellow Sea

Abstract

A Gram-negative, non-spore-forming, catalase- and oxidase-positive, strictly aerobic, short rod-shaped bacterium with a single, polar flagellum, designated strain WH169T, was isolated from seawater of the Yellow Sea in China. Buds and prosthecae were formed when the organism was grown at 20 °C for 12 days on marine 2216E agar. The organism grew optimally at 37 °C, in pH 7.0-8.0, and in the presence of 4.0-6.0% w/v NaCl. Growth did not occur in a medium without Na+ or sea salts. Strain WH169T contained ubiquinone-8 as the predominant respiratory lipoquinone and C 16:1 ω7c and/or C16:1 ω6c (35.9%), C 16:0 (25.3%) and C18:1 ω7c (9.7%) as the major fatty acids. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. The DNA G+C content of strain WH169T was 49.4 mol%. 16S rRNA gene sequence analysis showed that strain WH169T showed 95.1% sequence similarity to both type strains of the only two species in the genus Aestuariibacter. On the basis of the polyphasic taxonomic evidence presented in this study, it was concluded that strain WH169T should be classified as a novel species of Aestuariibacter, for which the name Aestuariibacter aggregatus sp. nov. is proposed, with the type strain WH169T (=CGMCC 1.8995T=LMG 25283T). © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.