In the past decade, laser microdissection (LMD) technology has been widely applied to plant tissues, high-lighting the role of different cell-type populations during plant–microbe interactions. In this chapter, a method to apply the LMD approach to study gene expression in specific cell-type populations of orchid mycorrhizal protocorms and roots is described in detail, starting from the preparation of biological material to gene expression analysis by RT-PCR.
CITATION STYLE
Balestrini, R., Fochi, V., Lopa, A., & Perotto, S. (2018). The Use of Laser Microdissection to Investigate Cell-Specific Gene Expression in Orchid Tissues (pp. 385–400). https://doi.org/10.1007/978-1-4939-7771-0_21
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