Objective: To assess the risk of hemolysis, disseminated intravascular coagulation (DIC), or fat embolism syndrome (FES) with pressurized intraosseous (10) blood transfusion following hemorrhage. Methods: A controlled, repeated‐measures, randomized animal study with blinded pathologic evaluations was conducted. Sixteen pento‐barbital‐anesthetized, instrumented immature swine underwent a 20‐mL/kg hemorrhage into citrate‐phosphate‐dextrose bags, then received autologous blood transfusion via a 16‐ga IV catheter (eight), or via a 15‐ga 10 needle in the proximal tibia (eight) under maximal manual pressure using a 30‐mL syringe. At baseline and at one hour and 48 hours posttransfusion, blood samples were assayed for hemoglobin (Hb), schistocytes, free Hb in plasma, bilirubin, lactate de‐hydrogenase, platelets, fibrinogen, and alveolar‐arteriolar O2 gradient. Lung sections were examined for inflammation after hematoxylid eosin stain, and for fat emboli after oil red‐O‐stain. Kidney sections were examined for inflammation using hematoxylin/eosin stain. Results: Though the 10 transfusion rate of 21 ± 6 mumin was slower than the IV rate of 35 ± 5 mL/min (p = 0.0012), all the animals returned to baseline blood pressure within 15 minutes and survived. The presence of schistocytes and mildly elevated free Hb in plasma was noted in both groups at baseline and each time period, and was presumed to be due to sampling from the arterial catheter. All other laboratory values remained within normal limits and without inter‐group differences at any time period. No fat embolus was noted, and all lung and kidney specimens were free of inflammation. Conclusions: In this model, pressurized 10 blood transfusion appears to be hematologically safe, i.e., without risk of appreciable hemolysis, DIC, or FES. © 1995 Society for Academic Emergency Medicine
CITATION STYLE
Plewa, M. C., King, R. W., Fenn‐Buderer, N., Gretzinger, K., Renuart, D., & Cruz, R. (1995). Hematologic Safety of Intraosseous Blood Transfusion in a Swine Model of Pediatric Hemorrhagic Hypovolemia. Academic Emergency Medicine, 2(9), 799–809. https://doi.org/10.1111/j.1553-2712.1995.tb03275.x
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