Alkali Comet Assay in Genotoxicity Tests

Abstract

DNA damage detected by the in vivo comet assay is an initial factor for Clastogenicity and gene mutation, and it is considered that the potential for carcinogenesis can be evaluated. However, there is a problem that the test results were not stable because the results fluctuated largely depending on the test execution conditions. Therefore, the Organisation for Economic Co-operation and Development (OECD) test guideline have described the conditions under which tests should be conducted in order to obtain stable data. Herein, I describe an in vivo comet assay that is based on recently approved the OECD test guideline (TG 489).