Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics

Abstract

Background: Migfilin is a focal adhesion protein implicated in control of integrin-mediated cell adhesion, spreading, and migration. Results: Migfilin LIM domains are required for focal adhesion targeting and for binding to kindlin-1 and kindlin-2. Conclusion: Kindlins support migfilin recruitment to focal adhesions and kindlin is important for normal migfilin dynamics in cells. Significance: Migfilin/kindlin interactions are important for regulating subcellular localization. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.