Supported lipid bilayers represent a very attractive way to mimic biological membranes, especially to investigate molecular mechanisms associated with the lateral segregation of membrane components. Observation of these model membranes with high-speed atomic force microscopy (HS-AFM) allows the capture of both topography and dynamics of membrane components, with a spatial resolution in the nanometer range and image capture time of less than 1 s. In this context, we have developed new protocols adapted for HS-AFM to form supported lipid bilayers on small mica disks using the vesicle fusion or Langmuir-Blodgett methods. In this chapter we describe in detail the protocols to fabricate supported artificial bilayers as well as the main guidelines for HS-AFM imaging of such samples.
CITATION STYLE
Nasrallah, H., Vial, A., Pocholle, N., Soulier, J., Costa, L., Godefroy, C., … Milhiet, P. E. (2019). Imaging artificial membranes using high-speed atomic force microscopy. In Methods in Molecular Biology (Vol. 1886, pp. 45–59). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8894-5_3
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