Circulating tumor cells (CTCs) hold promise as biomarkers to aid in patient treatment stratification and disease monitoring. Because the number of cells is a critical parameter for exploiting CTCs for predictive biomarker’s detection, we developed a FISH (fluorescent in situ hybridization) method for CTCs enriched on filters (filter-adapted FISH [FA-FISH]) that was optimized for high cell recovery. To increase the feasibility and reliability of the analyses, we combined fluorescent staining and FA-FISH and developed a semi-automated microscopy method for optimal FISH signal identification in filtration-enriched CTCs. Here we present these methods and their use for the detection and characterization of ALK-, ROS1-, RET-rearrangement in CTCs from non-small-cell lung cancer and ERG-rearrangements in CTCs from prostate cancer patients.
CITATION STYLE
Catelain, C., Pailler, E., Oulhen, M., Faugeroux, V., Pommier, A. L., & Farace, F. (2017). Detection of gene rearrangements in circulating tumor cells: Examples of ALK-, ROS1-, RET-rearrangements in non-small-cell lung cancer and ERG-rearrangements in prostate cancer. In Advances in Experimental Medicine and Biology (Vol. 994, pp. 169–179). Springer New York LLC. https://doi.org/10.1007/978-3-319-55947-6_9
Mendeley helps you to discover research relevant for your work.