Examining Amyloid Structure and Kinetics with 1D and 2D Infrared Spectroscopy and Isotope Labeling

Abstract

The combination of infrared spectroscopy and isotope labeling is a powerful toolset for providing residue-specific information about the structure and aggregation kinetics of amyloid peptides. In this chapter, we review a simple mathematical formalism to guide the interpretation of 1D- and 2DIR spectra of amyloid fibers. This formalism enables the design of isotope labeling schemes to extract precise structural features from IR spectra. We present 2DIR experiments on hIAPP and Aβ in which some of these strategies have been employed.