Digital Surface-Enhanced Raman Spectroscopy-Lateral Flow Test Dipstick: Ultrasensitive, Rapid Virus Quantification in Environmental Dust

11Citations
Citations of this article
11Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

This study introduces a novel surface-enhanced Raman spectroscopy (SERS)-based lateral flow test (LFT) dipstick that integrates digital analysis for highly sensitive and rapid viral quantification. The SERS-LFT dipsticks, incorporating gold-silver core-shell nanoparticle probes, enable pixel-based digital analysis of large-area SERS scans. Such an approach enables ultralow-level detection of viruses that readily distinguishes positive signals from background noise at the pixel level. The developed digital SERS-LFTs demonstrate limits of detection (LODs) of 180 fg for SARS-CoV-2 spike protein, 120 fg for nucleocapsid protein, and 7 plaque forming units for intact virus, all within <30 min. Importantly, digital SERS-LFT methods maintain their robustness and their LODs in the presence of indoor dust, thus underscoring their potential for accurate and reliable virus diagnosis and quantification in real-world environmental settings.

References Powered by Scopus

Optical constants of the noble metals

18419Citations
N/AReaders
Get full text

Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor

4456Citations
N/AReaders
Get full text

Characterization of a novel coronavirus associated with severe acute respiratory syndrome

2234Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Machine Learning-Assisted Surface-Enhanced Raman Spectroscopy Detection for Environmental Applications: A Review

4Citations
N/AReaders
Get full text

Pushing the Limits of Lateral Flow Immunoassay by Digital SERS for the Ultralow Detection of SARS-CoV-2 Virus

2Citations
N/AReaders
Get full text

Exploring Plasmonic Standalone Surface-Enhanced Raman Scattering Nanoprobes for Multifaceted Applications in Biomedical, Food, and Environmental Fields

1Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Wang, W., Srivastava, S., Garg, A., Xiao, C., Hawks, S., Pan, J., … Vikesland, P. J. (2024). Digital Surface-Enhanced Raman Spectroscopy-Lateral Flow Test Dipstick: Ultrasensitive, Rapid Virus Quantification in Environmental Dust. Environmental Science and Technology, 58(11), 4926–4936. https://doi.org/10.1021/acs.est.3c10311

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 1

50%

Researcher 1

50%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 2

40%

Physics and Astronomy 2

40%

Engineering 1

20%

Article Metrics

Tooltip
Mentions
Blog Mentions: 1

Save time finding and organizing research with Mendeley

Sign up for free