Bovine coronavirus (BCoV) is an economically significant cause of enteric and respiratory diseases in cattle throughout the world. BCoV is a known cause of neonatal calf diarrhea, winter dysentery in adult cattle, and respiratory disorders in cattle of all ages. In this chapter, we describe a simple and efficient protocol for total nucleic acids extraction to be used in conventional RT-PCR assay. This is a technique used routinely in our virology laboratory to detect BCoV from stool and nasopharyngeal samples of cattle.
CITATION STYLE
Alfieri, A. A., Alfieri, A. F., & Takiuchi, E. (2016). Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction (pp. 101–113). https://doi.org/10.1007/978-1-4939-3414-0_9
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