Enhancer trapping in plants.

Abstract

Advances in sequencing technology have led to the availability of complete genome sequences of many different plant species. In order to make sense of this deluge of information, functional genomics efforts have been intensified on many fronts. With improvements in plant transformation technologies, T-DNA and/or transposon-based gene and enhancer-tagged populations in various crop species are being developed to augment functional annotation of genes and also to help clone important genes. State-of-the-art cloning and sequencing technologies, which would help identify T-DNA or transposon junction sequences in large genomes, have also been initiated. This chapter gives a brief history of enhancer trapping and then proceeds to describe gene and enhancer tagging in plants. The significance of reporter gene fusion populations in plant genomics, especially in important cereal crops, is discussed.