New ultrasensitive sandwich-type immunoassay of dendritic Tri-Fan blade-like PdAuCu nanoparticles/amine-functionalized graphene oxide for label-free detection of carcinoembryonic antigen

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Abstract

The early detection of tumor markers has an effective role in the treatment of cancer. Here, a new sandwich-type electrochemical immunosensor for early label-free detection of the cancer biomarker carcinoembryonic antigen (CEA) was developed. Dendritic tri-fan blade-like PdAuCu nanoparticles (PdAuCu NPs)/amine functionalized graphene oxide (NH2-GO) were the label of secondary antibodies (Ab2), and Au nanoparticle-decorated polydopamines (Au/PDA) were immobilized on a screen-printed carbon electrode (SPCE) as the substrate materials. Dendritic tri-fan blade-like PdAuCu NPs/NH2-GO was synthesized according to a simple hydrothermal procedure and used to immobilize antibodies (Ab2) with large surfaces areas, increased catalytic properties and good adsorption to amplify the current signals. Subsequently, Ab2/PdAuCu NPs/NH2-GO catalyzed the reduction of H2O2 in the sandwich-type immunoreactions. Under optimal conditions, the immunosensor exhibited a satisfactory response to CEA with a limit detection of 0.07 pg mL−1 and a linear detection range from 0.1 pg mL−1 to 200 ng mL−1. The proposed immunosensor could be suitable enough for a real sample analysis of CEA, and has clinical value in the early diagnosis of cancer.

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Xu, P., Feng, W., Wang, M., Zhang, L., Liang, G., & Jing, A. (2021). New ultrasensitive sandwich-type immunoassay of dendritic Tri-Fan blade-like PdAuCu nanoparticles/amine-functionalized graphene oxide for label-free detection of carcinoembryonic antigen. Micromachines, 12(10). https://doi.org/10.3390/mi12101256

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