Matrix metalloproteinase, hyaluronidase and elastase inhibitory potential of standardized extract of Centella asiatica

Abstract

Context: Centella asiatica (L.) Urban (Apiaceae), a valuable herb described in Ayurveda, is used in the indigenous system of medicine as a tonic to treat skin diseases. Objective: Centella asiatica methanol extract and its ethyl acetate, n-butanol and aqueous fraction, were subjected for the evaluation of skin care potential through the in vitro hyaluronidase, elastase and matrix metalloproteinase-1 (MMP-1) inhibitory assay. Materials and methods: The C. asiatica plant was extracted with methanol and fractionated with ethyl acetate, n-butanol and water. The enzymatic activities were evaluated using ursolic acid and oleanolic acid as standards. Isolate molecule asiaticoside was quantified in the crude extract and fractions through high-performance liquid chromatography (HPLC) and structural was characterized by liquid chromatography-mass spectroscopy (LC-MS) and 1H nuclear magnetic resonance (NMR). Isolated compound was also evaluated for in vitro enzyme assays. Results: Extract exhibited anti-hyaluronidase and anti-elastase activity with IC 50 of 19.27 ± 0.37 and 14.54 ± 0.39 μg/mL, respectively, as compared to ursolic acid. Centella asiatica n-butanol fraction (CAnB) and isolated compound showed significant hyaluronidase (IC50 = 27.00 ± 0.43 and 18.63 ± 0.33 μg/mL) and elastase (IC 50 = 29.15 ± 0.31 and 19.45 ± 0.25 μg/mL) inhibitory activities, respectively, and also showed significant MMP-1 inhibition (p < 0.05 and p < 0.01). Discussion and conclusion: n-Butanol fraction was found to be most effective among the all fractions from which asiaticoside was isolated and further quantified by HPLC. This work concludes that the asiaticoside from C. asiatica may be a prospective agent for skin care. © 2013 Informa Healthcare USA, Inc.