A new robust diagnostic polymerase chain reaction for determining the mating status of female Anopheles gambiae mosquitoes

9Citations
Citations of this article
53Readers
Mendeley users who have this article in their library.

Abstract

The principal malaria vector in Africa, Anopheles gambiae, contains two pairs of autosomes and one pair of sex chromosomes. The Y chromosome is only associated with males and other Y chromosome-specific DNA sequences, which are transferred to women during mating. A reliable tool to determine the mating status of dried wild An. gambiae females is currently lacking. DNA was extracted from dried virgin and mated females and used to test whether Y chromosome-specific polymerase chain reaction (PCR) markers can be successfully amplified and used as a predictor of mating. Here we report a new PCR-based method to determine the mating status among successfully inseminated and virgin wild An. gambiae females, using three male-specific primers. This dissection-free method has the potential to facilitate studies of both population demographics and gene flow from dried mosquito samples routinely collected in epidemiologic monitoring and aid existing and new malaria-vector control approaches. Copyright © 2007 by The American Society of Tropical Medicine and Hygiene.

Cite

CITATION STYLE

APA

Ng’habi, K. R., Horton, A., Knols, B. G. J., & Lanzaro, G. C. (2007). A new robust diagnostic polymerase chain reaction for determining the mating status of female Anopheles gambiae mosquitoes. American Journal of Tropical Medicine and Hygiene, 77(3), 485–487. https://doi.org/10.4269/ajtmh.2007.77.485

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free