Integrated oxidative folding of cysteine/selenocysteine containing peptides: Improving chemical synthesis of conotoxins

Abstract

Building bridges: The use of diselenide and selectively ( 15N/13C)-labeled disulfide bridges is combined to give improvements in oxidative folding and disulfide mapping. Conotoxin analogues, each with a pair of selenocysteines (Sec) and labeled cysteines (see scheme, red), exhibited significantly improved folding and the labeled cysteines allow correctly folded species to be rapidly identified by NMR spectroscopy. (Chemical Equation Presented) © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.