Measurement of metabolite changes in senescent cells by mass spectrometry

Abstract

Senescent cells display altered metabolic signatures that can either be causal for aspects the senescent phenotype, or act as biomarkers for senescence. Steady state levels of multiple metabolites change with senescence, and can be detected using analytical methods. Here, we describe a liquid chromatography–mass spectrometry (LC-MS) method for detecting altered metabolites from cultured senescent cells.