Flow cytometry, a universe of possibilities in the veterinary field. Review

Abstract

Flow cytometry is a technology that has helped to rapidly advance many diverse areas of science by allowing the simultaneous measurement of multiple characteristics of each of the individual particles or cells in a sample as they pass at high speed through an area illuminated by one or more lasers. The information obtained includes data on the size and internal complexity, as well as other parameters inherent to each of the particles present in the sample, which are captured by the equipment as light signals. The most common particles analyzed in flow cytometers are cells, so the expression of molecules on their surface and inside, viability, functionality, cell proliferation, DNA content, cytokine production and many others can be analyzed. These determinations can be carried out by using antibodies coupled to fluorochromes or by using molecules whose fluorescence depends on the characteristic to be evaluated. Some flow cytometers are also sorters, which means that the equipment can physically sort those cells that exhibit the characteristics of interest; in addition, it is feasible that once they have been purified, they can be used in subsequent experiments. This review focuses on the fundamentals of flow cytometry and its main applications, which offer a great window of opportunity in the veterinary field, both in research and in the clinic.