HPLC/DAD/ESI-MS analysis of non-volatile constituents of three Brazilian chemotypes of Lippia alba (Mill.) N. E. Brown

Abstract

Aqueous preparations and ethanolic extracts of three Brazilian chemotypes of Lippia alba (Mill.) N.E. Brown (Verbenaceae) were investigated for the chemical variability of their non volatile constituents by HPLC/DAD/ESI-MS analysis. The main class of compounds in all the extracts investigated was phenylpropanoids, mainly verbascoside, followed by the flavonoids tricin-7-O-diglucuronide (present in Lippia alba chemotypes 2 and 3), luteolin-7-O-glucuronide (present in L. alba chemotype 1) and mono-and di-O-glucuronic derivatives of apigenin and tricin. Four iridoids, geniposidic acid, theveside, 8-epi-loganin and mussaenoside were also identified.