Quantitative real-time PCR detection of airborne Staphylococcus aureus in hospital indoor atmosphere

Abstract

Staphylococcus aureus is a classical type of gram-positive bacteria. It is attached to skin, mucous membranes, especially pharynx nasalis, as an opportunistic pathogen which can cause a wide variety of infectious diseases. With increasing use of antibiotic the resistance of Staphylococcus aureus is increased. And more and more methicillin-resistant Staphylococcus aureus which caused seriously disease emerged recent years. So a sensitive, rapid, and specific method was developed using real-time PCR to detect the number of Staphylococcus aureus survived in atmosphere of a specific respiration ward. In this study thirteen samples were collected and assessed in all during the second quarter of 2011.