The Genome Assembly of Vitis vinifera cv. Shiraz

2Citations
Citations of this article
17Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Background and Aims. Shiraz (Syrah) is a dark-skinned cultivar of the wine grape Vitis vinifera that forms the basis of some of the world's most iconic wines. Worldwide, Shiraz is the fourth most planted grapevine cultivar; however, it represents the most planted cultivar in Australia. Given the importance of Shiraz to worldwide wine production, this study aimed to produce a reference genome for this cultivar while investigating the unique genetic variants and ancestral origins of this iconic variety. Methods and Results. Long-read ONT data were selected to produce a highly contiguous genome assembly for Shiraz. Phylogenetic reconstruction using high-quality genome assemblies for wine grape cultivars provided further support of a kinship between Shiraz and Pinot Noir. Harnessing long-read data, transposable element insertions potentially affecting gene function were characterized in Shiraz and assessed relative to other cultivars. This revealed a heterogenous landscape of transposon insertion points across cultivars and uncovered a specific combination of allelic variants at the VviTPS24 terpene synthase locus. Conclusions. This establishment of a Shiraz genome provides a detailed view of the genetics that underpin this cultivar, including the discovery of a specific combination of VviTPS24 variants, which when combined with appropriate environmental triggers may allow Shiraz to produce high levels of rotundone, the aroma compound responsible for the distinctive peppery characteristics of this cultivar. Significance of the Study. The availability of a reference genome for Shiraz expands the pool of genomes available for wine grapes while providing a foundation resource for whole-genome studies involving this iconic cultivar, including intracultivar variant identification and transcriptomic studies using a matching reference genome.

References Powered by Scopus

The Sequence Alignment/Map format and SAMtools

41091Citations
N/AReaders
Get full text

MUSCLE: Multiple sequence alignment with high accuracy and high throughput

35681Citations
N/AReaders
Get full text

BEDTools: A flexible suite of utilities for comparing genomic features

16304Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Integrated omics-based exploration for temperature stress resilience: An approach to smart grape breeding strategies

6Citations
N/AReaders
Get full text

Diploid genome assembly of the Malbec grapevine cultivar enables haplotype-aware analysis of transcriptomic differences underlying clonal phenotypic variation

4Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Onetto, C. A., Ward, C. M., & Borneman, A. R. (2023). The Genome Assembly of Vitis vinifera cv. Shiraz. Australian Journal of Grape and Wine Research, 2023. https://doi.org/10.1155/2023/6686706

Readers over time

‘23‘24‘25036912

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 2

40%

Researcher 2

40%

Lecturer / Post doc 1

20%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 4

67%

Biochemistry, Genetics and Molecular Bi... 2

33%

Article Metrics

Tooltip
Social Media
Shares, Likes & Comments: 3

Save time finding and organizing research with Mendeley

Sign up for free
0