Bacteria have developed a number of trans-envelope systems to transport molecules or assemble organelles across bacterial envelopes. However, bacterial envelopes contain a rigid netlike peptidoglycan structure that protects cells from osmotic lysis. Trans-envelope systems thus must interact with the peptidoglycan barrier to generate gaps or anchor structures to the peptidoglycan scaffold. Here we describe methods to use in vivo cross-linking and in vitro co-sedimentation to study protein–peptidoglycan interactions in Gram-negative bacteria. In particular, we address important considerations to ensure the specificity of the interactions in question.
CITATION STYLE
Li, G., & Peter Howard, S. (2017). In vivo and in vitro protein–peptidoglycan interactions. In Methods in Molecular Biology (Vol. 1615, pp. 143–149). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7033-9_11
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