Iodine-125 radioprobing is based on incorporation of radioiodine into a defined position in a nucleic acid molecule. Decay of (125)I results in the emission of multiple, low-energy Auger electrons that, along with positively charged residual daughter nuclide, produce DNA strand breaks. The probability of such strand breaks at a given nucleotide is in inverse proportion to the distance from the (125)I atom to the sugar of that nucleotide. Therefore, conclusions can be drawn about the conformation or folding of a DNA or RNA molecule based on the distribution of (125)I decay-induced strand breaks. Here we describe in detail the application (125)I radioprobing for studying the conformation of quadruplex structures, and discuss the advantages and limitations of the method.
CITATION STYLE
Gaynutdinov, T. I., Neumann, R. D., & Panyutin, I. G. (2010). Assessing DNA structures with 125I radioprobing. Methods in Molecular Biology (Clifton, N.J.), 608, 137–145. https://doi.org/10.1007/978-1-59745-363-9_9
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