Plant Regeneration by Organogenesis from Strawberry Leaf and Runner Tissue

Abstract

Methods were developed for efficient shoot regeneration from leaf and runner tissues of the strawberry ( Fragaria × ananassa ) cultivars Allstar and Honeoye. Optimal regeneration conditions differed for ‘Allstar’, depending on whether leaf tissue was derived from plantlets grown in vitro or from plants grown in the greenhouse. ‘Allstar’ leaf tissues derived from in vitro cultures regenerated shoots most efficiently in a Linsmaier–Skoog (LS) medium containing 2.5 mg BA and 0.5 mg IBA/liter. ‘Allstar’ leaf tissues derived from greenhouse plants regenerated shoots best in LS medium containing 3.0 mg BA and 0.1 mg IBA/liter. Addition of casein hydrolysate (CH) at either 400 or 600 mg·liter −1 stimulated shoot production. A supplement of KNO 3 at 2000 mg·liter −1 also enhanced regeneration efficiency of greenhouse-grown leaf tissues. ‘Honeoye’ had lower regeneration potential in most treatments than ‘Allstar’ and only produced shoots in a LS medium containing 5.0 mg BA, 0.5 mg IBA, and 400 mg CH/liter. Shoots from runner tissues of both cultivars were best obtained using LS medium containing 10.0 mg BA, 2.0 IAA, and 500 mg CH/liter. Shoot production from runners was dependent on the diameter of the runner, with diameters of <2.0 mm having poor regeneration potential. Chemical names used: N -(phenylmethyl)-1 H -purin-6-amine (BA); 1 H -indoIe-3-butyric acid (IBA); and 1 H -indole-3-acetic acid (IAA).