At Weyerhaeuser Company, somatic embryo production in liquid medium and manufactured seed delivery has been developed to reduce labor costs and increase efficiency of mass clonal propagation. We have scaled-up embryonal suspensor masses (ESM) of Douglas-fir in 1-litre liquid medium flasks. Over 10,000 somatic embryos have been produced from a single flask. We have cryostored ESM of over 700 genotypes in liquid nitrogen. Somatic embryos of Douglas-fir have also been produced from liquid medium in a bioreactor. Different types of bioreactors are required for embryo multiplication and for cotyledonary embryo development. Over 250,000 Douglas-fir somatic seedlings from a large number of genotypes have been produced for clonal field tests.
CITATION STYLE
Gupta, P. K., & Timmis, R. (2005). Mass propagation of conifer trees in liquid cultures - Progress towards commercialization. In Liquid Culture Systems for in vitro Plant Propagation (Vol. 9781402031991, pp. 389–402). Springer Netherlands. https://doi.org/10.1007/1-4020-3200-5_30
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