Effect of high concentrations of sucrose on the enzymatic activity of α-chymotrypsin

Abstract

The effect of the low-molecular-mass natural reagents in high concentrations is important for investigating enzymatic reactions in near 'in vivo' conditions and for optimisation of biotechnology processes. A model system, including p-nitrophenyl acetate as substrate and α-chymotrypsin as proteolytic enzyme, has been used to study the effect of high concentrations of sucrose, both influencing the viscosity of the reaction medium and acting as a nucleophilic effector (activator) on the enzymatic reaction. A kinetic scheme at high concentrations of nucleophilic effectors (sucrose) has been proposed.