Background: The neuroprotective effect of xenon has been demonstrated for glutamatergic neurons. In the present study it is investigated if dopaminergic neurons, i.e. nerve-growth-factor differentiated PC-12 cells, are protected as well against hypoxia-induced cell damage in the presence of xenon. Results: Pheochromocytoma cells differentiated by addition of nerve growth factor were placed in a N2-saturated atmosphere, a treatment that induced release of dopamine, reaching a maximum after 30 min. By determining extracellular lactate dehydrogenase concentration as marker for concomitant cellular damage, a substantial increase of enzymatic activity was found for N2-treated cells. Replacement of N2 by xenon in such a hypoxic atmosphere resulted in complete protection against cellular damage and prevention of hypoxia-induced dopamine release. Intracellular buffering of Ca2+ using the Ca-chelator 1, 2-bis(2-Aminophenoxy ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester (BAPTA) reduced the neuroprotective effect of xenon indicating the essential participation of intracellular Ca2+-ions in the process of xenon-induced neuroprotection. Conclusions: The results presented demonstrate the outstanding property of xenon to protect neuron-like cells in a hypoxic situation. © 2004 Petzelt et al; licensee BioMed Central Ltd.
CITATION STYLE
Petzelt, C., Blom, P., Schmehl, W., Müller, J., & Kox, W. J. (2004). Xenon prevents cellular damage in differentiated PC-12 cells exposed to hypoxia. BMC Neuroscience, 5. https://doi.org/10.1186/1471-2202-5-55
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