High-Throughput Direct Cell Counting Method for Immuno-Oncology Functional Assays Using Image Cytometry

Abstract

The last decade has seen significant progress in the field of immuno-oncology. The increase in new technologies has led to the development of novel immuno-oncology assays to investigate immune responses to cancer cells. Numerous cell-based assays have utilized image cytometry due to its abilities to perform high-throughput screening, observe and automatically analyze cells directly in standard microplates, and digitally store the acquired cell images. In this chapter, we describe the use of Celigo Image Cytometer for immuno-oncology functional assays such as complement-dependent cytotoxicity (CDC), antibody-dependent cell-mediated cytotoxicity (ADCC), T cell proliferation, and T cell migration.