A Simple RP-HPLC Method for Estimation of Triclabendazole and Ivermectin in a Pharmaceutical Suspension Dosage Form.

Abstract

A simple method has been developed for the estimation of triclabendazole and ivermectin in a pharmaceutical suspension dosage form present in 50:1 ratio respectively, by reversed-phase High-Performance Liquid Chromatography (HPLC). The isocratic LC analysis was performed on Vydac, C-18, (250 X 4.6 mm, 5 µ) using mobile phase comprising of acetonitrile, methanol and water at a flow rate of 1.5 ml/minute. Quantification was carried out by using UV detector at 254 nm and the run time was 15 minutes. Linearity was found to be 0.06-0.14 and 0.03-0.05 mg/ml with a correlation coefficient , r= 0.998 and 0.999 for triclabendazole and ivermectin respectively. The percentage recovery values were found to be within the range of 98-102. The analytical method has been successively applied to pharmaceutical formulation and was validated according to International Conference on Harmonization (ICH) guidelines.