The CCAAT Enhancer-binding Protein α (C/EBPα) Requires a SWI/SNF Complex for Proliferation Arrest

Abstract

The transcription factor CCAAT enhancer-binding protein α (C/EBPα) is a tumor suppressor in myeloid cells and inhibits proliferation in all cell types examined. C/EBPα interacts with the SWI/SNF chromatin-remodeling complex during the regulation of differentiation-specific genes. Here we show that C/EBPα fails to suppress proliferation in SWI/SNF defective cell lines after lmock-down of SWI/SNF core components or after deletion of the SWI/SNF interaction domain in C/EBPα, respectively. Reconstitution of SWI/SNF function restores C/EBPα-dependent proliferation arrest. Our results show that the anti-proliferation activity of C/EBPα critically depends on components of the SWI/SNF core complex and suggest that the functional interaction between SWI/SNF and C/EBPα is a prerequisite for proliferation arrest.