Radiosensitization of breast cancer cells by TRAIL-endostatin-targeting gene therapy

11Citations
Citations of this article
9Readers
Mendeley users who have this article in their library.

Abstract

One of the key issues in cancer radiotherapy research is to sensitize tumor cells to the cell killing effects of ionizing radiation while leaving normal tissues intact. One potential approach to achieve this is gene-radiotherapy, i.e. a combination of radiation therapy and gene therapy. It is to choose certain exogenous radiation-inducible regulatory genes, for example, early growth response-1 (Egr-1), and transcript its downstream tumor-therapeutic genes under ionizing radiation so as to kill the tumor cells synergistically by the expressed gene products together after transfection and irradiation exposure. In this study, we engineered a plasmid encoding both TRAIL and endostatin under the control of the radiation-inducible Egr-1 promoter, and evaluated its anti-tumor efficacy in combination with radiotherapy. Our plasmid showed significant efficacy in up-regulating the levels of TRAIL and endostatin proteins after transfected into breast cancer cells and exposed to X-ray irradiation. The detected cellular effects in vitro manifested that TRAIL-endostatin-based gene therapy could enhance radiosensitizing effects in breast cancer cells in terms of tumor cell growth inhibition, promoting apoptosis and the induction of cell cycle arrest. In summary, our results suggest that TRAIL-endostain-targeting approach might be a promising method to sensitize solid tumors to radiation therapy.

References Powered by Scopus

Cancer statistics, 2009

10121Citations
N/AReaders
Get full text

Endostatin: An endogenous inhibitor of angiogenesis and tumor growth

4360Citations
N/AReaders
Get full text

Identification and characterization of a new member of the TNF family that induces apoptosis

2735Citations
N/AReaders
Get full text

Cited by Powered by Scopus

EGR-1/Bax pathway plays a role in vitamin E δ-tocotrienol-induced apoptosis in pancreatic cancer cells

47Citations
N/AReaders
Get full text

Inhibiting effect of Endostar combined with ginsenoside Rg3 on breast cancer tumor growth in tumor-bearing mice

38Citations
N/AReaders
Get full text

The Clinical Research of Serum VEGF, TGF-β1, and Endostatin in Non-small Cell Lung Cancer

16Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Li, Y. B., Guo, C. X., Wang, Z. C., Dong, L. H., Guan, F., Liu, Y., … Gong, S. L. (2013). Radiosensitization of breast cancer cells by TRAIL-endostatin-targeting gene therapy. Neoplasma, 60(6), 613–619. https://doi.org/10.4149/neo_2013_079

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 2

67%

Lecturer / Post doc 1

33%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 2

40%

Computer Science 1

20%

Immunology and Microbiology 1

20%

Psychology 1

20%

Save time finding and organizing research with Mendeley

Sign up for free