Molecular diversity pattern of intestinal lactic acid bacteria in Cemani chicken, Indonesian native chicken, as revealed by terminal restriction fragment length polymorphisms

Abstract

See, stats, and : https : / / www . researchgate . net / publication / 298792445 Molecular acid , Indonesian native , as . . . . Article DOI : 10 . 21161 / mjm . 73815 CITATIONS 0 READS 104 4 , including : Some : 1 . Multilocation biofertilizer Nitrogen Achmad Indonesian 16 SEE K . G . Wiryawan Bogor 132 SEE Iman Bogor 89 SEE All . The .ABSTRACTAims:AnecologicalstudywasconductedtoinvestigatethediversitypatternofthelacticacidbacteriainthegastrointestinaltractofCemanichicken,anativeIndonesianchicken,usingamolecularapproachbasedon16SrRNAgenes.Methodologyandresults:Digestasamplesofsevenchickenswerecollectedforterminalrestrictionfragmentlengthpolymorphism(T-RFLP)analysis.Themoleculardiversityoflacticacidbacteriaincrop,ventriculus,ileumandcecumweredetermined.Theresultsshowedthatmicrobialcompositionoflacticacidbacteriaincecumwasrelativelydifferentwithotheruppergastrointestinaltract.Lacticacidbacteriaphylotypesanddiversityinileumwerehigherthanthoseinthecrop,ventriculus,andcecum.Conclusion,significanceandimpactofstudy:WeconfirmedthatcecumofnativechickenhasadifferentenvironmentascomparedtoothergastrointestinalregionsshowingthelowestvalueoftheSorensen'sindex.ThisfirstreportofLABdiversitypatterninCemanichickencontributesamorecomprehensiveunderstandingofthemicrobialecologyinthechicken.